Journal: Nature Communications

Article Title: Polystyrene nanoplastics disrupt the intestinal microenvironment by altering bacteria-host interactions through extracellular vesicle-delivered microRNAs

doi: 10.1038/s41467-025-59884-y

Figure Lengend Snippet: A Alcian blue-periodic acid Schiff (AB-PAS) staining of intestinal mucus in mice with or without NP treatment. Scale bars indicate 100 µm (upper) and 50 µm (lower). Data were shown as mea n ± SEM ( n = 12). B IHC stain of intestinal MUC-13 in NP-treated mice. Scale bars indicate 100 µm (upper) and 50 µm (lower). Data were shown as mea n ± SEM ( n = 12) (*** p value < 0.001). C MUC13 expression in NP-treated enterocyte-like differentiated Caco-2 cells for 48 h by ICC stain (Scale bars indicate 20 µm), qPCR, and Western blot. Data are presented as means ± SD ( n = 3). Significant difference was shown by different letters (* p < 0.05; *** p < 0.001). D Western blot analysis of MUC-13 levels in goblet-like LS174T cells treated with NP for 48 h. E Heatmap predicting various intestinal miRNAs suppressing MUC-13 in NP-exposed mice. Validation of miR-700-5p interference on MUC-13 in enterocyte-like differentiated Caco-2 cells by ( F ) qPCR. G ICC stain (Scale bars indicate 20 µm), and ( H ) Western blot. Data are presented as means ± SD ( n = 3). Significant difference was shown by different letters (*** p < 0.001). I Schematic of NP im p act on MUC-13 and mucus secretion in the gut.

Article Snippet: MUC13 (bs-10074R, Bioss, Boston, Massachusetts, USA) expression in enterocyte-like differentiated Caco-2 cells, goblet-like LS174T cells, and mice was evaluated by immunocytochemistry staining.

Techniques: Staining, Expressing, Western Blot, Biomarker Discovery

Journal: Nature Communications

Article Title: Polystyrene nanoplastics disrupt the intestinal microenvironment by altering bacteria-host interactions through extracellular vesicle-delivered microRNAs

doi: 10.1038/s41467-025-59884-y

Figure Lengend Snippet: A The effects of NP treatment (1 × 10 10 particles/mL) on the growth of various lactic acid bacteria ( L. paracasei, L. acidophilus , and P. acidiloctici ) , Lachnospiraceae sp. (TSD-26; ATCC), and Ruminococcaceae sp. (TSD-27; ATCC). B Schematic of experimental process by interactions between bacterial EV and cell-derived EV. C Impact of Lachnospiraceae sp.-derived EV without or with NP treatment (1 × 10 10 particles/mL) for 18 h on the growth of different bacterial species ( L. paracasei , L. acidophilus , P. acidiloctici , and Ruminococcaceae sp.). D The impact of Ruminococcaceae sp.-derived EV without or with NP treatment (1 × 10 10 particles/mL) for 44 h on the growth of different bacterial species ( L. paracasei , L. acidophilus , P. acidiloctici , and Lachnospiraceae sp.). E Impact of goblet-like LS174T cells without or with NP treatment (10 6 particles/mL) for 48 h on the growth of different bacterial species ( L. paracasei, L. acidophilus, P. acidiloctici , and Lachnospiraceae sp. and Ruminococcaceae sp.). F Western blot of MUC13 inhibition by Lachnospiraceae sp.-derived EV. Data were shown as mea n ± SD ( n = 3) (* p value < 0.05). G Schematic representation summarizing the proposed mechanisms of NP-induced modulation of gut microbiota via EV. NP are taken up by Lachnospiraceae , whose EV suppress MUC13 expression in goblet cells. Concurrently, NP-modified EV from goblet cells promote the growth of Ruminococcaceae , collectively contributing to gut microbiota imbalance and potential intestinal barrier dysfunction.

Article Snippet: MUC13 (bs-10074R, Bioss, Boston, Massachusetts, USA) expression in enterocyte-like differentiated Caco-2 cells, goblet-like LS174T cells, and mice was evaluated by immunocytochemistry staining.

Techniques: Bacteria, Derivative Assay, Western Blot, Inhibition, Expressing, Modification

Journal: The Journal of Cell Biology

Article Title: BEACH domain proteins function as cargo-sorting adaptors in secretory and endocytic pathways

doi: 10.1083/jcb.202408173

Figure Lengend Snippet: Antibodies

Article Snippet: MUC13 antibody , Cell Signaling Technology , Cat# 44454.

Techniques:

Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

Article Title: Alterations in cellular metabolic pathway and epithelial cell maturation induced by MYO5B defects are partially reversible by LPAR5 activation

doi: 10.1152/ajpgi.00091.2024

Figure Lengend Snippet: Lack of effect of Compound-1 on MUC13 localization in MVID model mice. Immunostaining for MUC13 and ACTG1 in jejunal tissues of control, MYO5BΔIEC, and MYO5B(G519R) mice treated with Compound-1 or vehicle. In control jejunum and distal colon, MUC13 (red) is localized to the apical tips of microvilli above actin filaments (green). Both MYO5B loss-of-function mouse tissues demonstrate strong MUC13 immunostaining in cytoplasm of jejunal and colonic epithelial cells. The mislocalization of MUC13 is not remarkably altered by Compound-1 treatments in MYO5BΔIEC or MYO5B(G519R) mice. Scale bars = 20 µm. MYO5B, myosin Vb; MVID, microvillus inclusion disease.

Article Snippet: The primary antibodies against NDUFB8 (1:1,000, Abcam ab192878), HMGCS2 (1:50, Abcam ab137043), NHE3 (1:200, Novus NBP1-82574), SGLT1 (0.5 μg/mL, own) , LAMP1 (1:100, Santa Cruz sc-19992), PHH3 (1:50, Novus NBP3-08511IR, DyLight 750), Ki67 (1:100, Cell Signaling 11882S, Alexa Fluor 488), DCLK1 (1:2,000, Abcam ab202755 Alexa Fluor 647), PCNA (1:50, Santa Cruz sc-56 Alexa Fluor 647), β-catenin/CTNNB1 (12F7) (1:100, Novus NBP1-54467R, DyLight 550), villin (1:50, Santa Cruz sc-58897 Alexa Fluor 488), MUC13 (1:100, Santa Cruz, sc-390115 Alexa Fluor 546), and ACTG1 (1:100, Santa Cruz sc-65638 Alexa Fluor 546 or Alexa Fluor 790) were diluted in Dako antibody diluent with background reducing compound (S3022) and incubated on sections for 1 h at room temperature or overnight at 4°C.

Techniques: Immunostaining, Control